Concomitantly, COL, EZ, CH, HQ, DZ, CD and VBL induced meiotic delay in primary and/or secondary spermatocytes. DZ and CD were less effective but significantly positive ( P < 0.05). COL, EZ, CH, HQ and VBL clearly increased the frequencies of hyperploid secondary spermatocytes which indicated non-disjunction induction during the first meiotic division. The proportions of spermatogonial mitoses, first and second meiotic metaphases were determined in order to recognize an effect of the test chemicals on testicular cell proliferation. Secondary spermatocytes of mice were evaluated by chromosome counting to determine the induction of hyperploidy. Two different criteria were used for the evaluation of slides from testicular material of (102/E1 × C3H/El)F 1 mice at different times (6, 14 and 22 h) after treatment with different doses of each of the test chemicals. The 10 known and suspect spindle poisons colchicine (COL), econazole (EZ), chloral hydrate (CH), hydroquinone (HQ), diazepam (DZ), thiabendazole (TB), cadmium chloride (CD), pyrimethamine (PY), thimerosal (TM) and vinblastine (VBL) were tested for aneuploidy induction in male germ cells.
Assays for aneuploidy are being developed within a coordinated research program sponsored by the Commission of the European Communities.
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